Questions? DIG DNA Labeling Mix is an easy-to-use labeling mixture for rapid random-primed labeling with digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP). The mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. Sample Materials As template for the labeling reaction • DNA fragments of at least 100bp• Linearized plasmids, cosmid or λDNA,• Supercoiled DNA,• Or minimal amounts of DNA (10ng), e.g., DNA restriction fragments isolated from low melting point agarose can be used.Note: Linear DNA is labeled more efficiently than circular and supercoiled DNA. Site Use Terms
UNG is active on single- and double-stranded dU-containing DNA, but dUTP is not a substrate for UNG. Type in Product Names, Product Numbers, or CAS Numbers to see suggestions.
Contains 0.25 ml of 100 mM ultrapure dUTP. Customer Service. The complementary DNA strand is synthesized by Klenow enzyme which uses the 3′-OH termini of the random oligonucleotides as primers and a mixture of deoxyribonucleotides containing DIG-11-dUTP, alkali-labile, for elongation. All Rights Reserved. Roche DIG labeling and detection reagents and kits, greener alternative product characteristics. Need larger quantities for your development, manufacturing or research applications? Lot and Batch Numbers can be found on a product's label number 09019TO without the first two letters. search was unable to find the COA you are looking for, you will be 05427ES–021 - enter the lot number 05427ES without the filling-code The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Not for use in diagnostic procedures. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. If you find a lot number such as TO09019TO - enter the lot Figure 7: If dUTP:dTTP increases, DNA polymerase frequently incorporates uracil instead of thymine during both replication and repair. STBB0728K9 - enter the lot number STBB0728 without the filling-code | Privacy. This density of haptens in the DNA yields the highest sensitivity in the detection reaction. Carryover of previously amplified PCR products is the single most significant source of contamination in subsequent PCR. you enter 062K1064. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others. It is designed … All nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Uracil-DNA glycosylase removes the uracil and initiates further repair involving DNA strand breaks in an intermediate step. Function-tested in the DIG DNA Labeling Kit and in the DIG Nucleic Acid Detection Kit. Deoxyuridine triphosphate, also known as deoxy-UTP or dUTP, belongs to the class of organic compounds known as pyrimidine 2'-deoxyribonucleoside triphosphates. presented with a COA Request form.
Only carryover product will be removed with UNG treatment.
Repair synthesis, however, may reintroduce uracil, leading to a futile DNA repair cycle. This density of haptens in the DNA yields the highest sensitivity in the detection reaction. If your
A nucleoside triphosphate is a molecule containing a nitrogenous base bound to a 5-carbon sugar (either ribose or deoxyribose), with three phosphate groups bound to the sugar. Pricing & availability is not currently available. Thermo Scientific dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM. New PCR product, amplified from authentic DNA template, though containing dUTP, is not … DIG DNA Labeling Mix is an easy-to-use labeling mixture for rapid random-primed labeling with digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP). Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR. Taq polymerase and other components of the PCR mixture are not affected by UNG treatment. It is an example of a nucleotide.They are the molecular precursors of both DNA and RNA, which are chains of nucleotides made through the processes of DNA replication and transcription.
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